Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31486
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dc.contributor.authorGalindo, Anaen_UK
dc.contributor.authorGarrido, Diegoen_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorPerez, Jose Aen_UK
dc.contributor.authorBetancor, Monicaen_UK
dc.contributor.authorAcosta, Nieves Gen_UK
dc.contributor.authorKabeya, Naokien_UK
dc.contributor.authorMarrero, Manuelen_UK
dc.contributor.authorBolanos, Anaen_UK
dc.contributor.authorRodriguez, Covadongaen_UK
dc.date.accessioned2020-07-25T00:04:53Z-
dc.date.available2020-07-25T00:04:53Z-
dc.date.issued2021-01-15en_UK
dc.identifier.other735761en_UK
dc.identifier.urihttp://hdl.handle.net/1893/31486-
dc.description.abstractReducing the dependency of fishfeed for marine ingredients and species diversification are both considered crucial factors for the sustainable development of aquaculture. The substitution of fish oil (FO) by vegetable oils (VO) in aquafeeds is an economically feasible solution. However, such substitution may compromise the fish flesh content of essential n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) and, therefore, its nutritional value for human consumption. Likewise, there is a wide range of strategies to select new target species for sector diversification, among which, the capacity to biosynthesize n-3 LC-PUFA from their C18 precursors abundant in VO might be considered as a fair preliminary strategy. Therefore, the aim of the present study was to analyze the metabolic fate of [1-14C] labeled 18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3 in isolated hepatocytes and enterocytes from wild individuals of three fish species with different trophic level: the marine herbivorous salema (Sarpa salpa), the strict carnivorous sand sole (Pegusa lascaris) and the omnivorous thicklip grey mullet (Chelon labrosus). These species were selected for their phylogenetic proximity to consolidated farmed species such as gilthead seabream (Sparus aurata), senegalese sole (Solea senegalensis), and golden grey mullet (Liza aurata), respectively. The study also assessed the molecular cloning, functional characterization and tissue distribution of the fatty acyl elongase (Elovl) gene, elovl5, involved in the biosynthetic metabolism of n-3 LC-PUFA. The three species were able to biosynthesize docosahexaenoic acid (22:6n-3). S. salpa seems to have similar biosynthetic capacity than S. aurata, with a fatty acyl desaturase 2 (Fads2), with 6, 8 and 5 activities. P. lascaris showed a wider Fads2 activity repertory than S. senegalensis, including 4 and residual 6/5 activities. In C. labrosus, both 8 and 5 activities but not the 6 described for L. aurata were detected in the incubated cells. Elongation from C18 and C20 precursors to C20 and C22 products occurred in hepatocytes and enterocytes as well as in the functional characterization of Elovl5 by heterologous expression in yeast. Elovl5 showed a species specific expression pattern, with the highest rates observed in the liver, gut and brain in S. salpa and P. lascaris, and in the brain for C. labrosus. In summary, the LC-PUFA biosynthesis capacity from S. salpa, P. lascaris and C. labrosus greatly resembled that of their phylogenetic closer species. The three studied species could be further explored as candidates for the aquaculture diversification from their potential ability to biosynthesize LC-PUFA.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationGalindo A, Garrido D, Monroig O, Perez JA, Betancor M, Acosta NG, Kabeya N, Marrero M, Bolanos A & Rodriguez C (2021) Polyunsaturated fatty acid metabolism in three fish species with different trophic level. Aquaculture, 530, Art. No.: 735761. https://doi.org/10.1016/j.aquaculture.2020.735761en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Galindo A, Garrido D, Monroig O, Perez JA, Betancor M, Acosta NG, Kabeya N, Marrero M, Bolanos A & Rodriguez C (2021) Polyunsaturated fatty acid metabolism in three fish species with different trophic level. Aquaculture, 530, Art. No.: 735761. https://doi.org/10.1016/j.aquaculture.2020.735761 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectChelon labrosusen_UK
dc.subjectElovl5en_UK
dc.subjectLC-PUFAen_UK
dc.subjectPegusa lascarisen_UK
dc.subjectSarpa salpaen_UK
dc.titlePolyunsaturated fatty acid metabolism in three fish species with different trophic levelen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2021-07-31en_UK
dc.rights.embargoreason[Final manuscript-Figures.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.aquaculture.2020.735761en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume530en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderMinistry of Economy and Competitiveness (Spain)en_UK
dc.author.emailm.b.betancor@stir.ac.uken_UK
dc.citation.date30/07/2020en_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationTokyo University of Marine Science and Technologyen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.identifier.isiWOS:000582169700058en_UK
dc.identifier.scopusid2-s2.0-85089285442en_UK
dc.identifier.wtid1647561en_UK
dc.contributor.orcid0000-0003-1626-7458en_UK
dc.date.accepted2020-07-22en_UK
dcterms.dateAccepted2020-07-22en_UK
dc.date.filedepositdate2020-07-23en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorGalindo, Ana|en_UK
local.rioxx.authorGarrido, Diego|en_UK
local.rioxx.authorMonroig, Oscar|en_UK
local.rioxx.authorPerez, Jose A|en_UK
local.rioxx.authorBetancor, Monica|0000-0003-1626-7458en_UK
local.rioxx.authorAcosta, Nieves G|en_UK
local.rioxx.authorKabeya, Naoki|en_UK
local.rioxx.authorMarrero, Manuel|en_UK
local.rioxx.authorBolanos, Ana|en_UK
local.rioxx.authorRodriguez, Covadonga|en_UK
local.rioxx.projectProject ID unknown|Ministry of Economy and Competitiveness (Spain)|en_UK
local.rioxx.freetoreaddate2021-07-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2021-07-30en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2021-07-31|en_UK
local.rioxx.filenameFinal manuscript-Figures.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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