Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31333
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPeleteiro, Maria Da Conceicao C V-
dc.date.accessioned2020-06-26T10:58:50Z-
dc.date.available2020-06-26T10:58:50Z-
dc.date.issued1987-
dc.identifier.urihttp://hdl.handle.net/1893/31333-
dc.description.abstractA series of studies concerning the structure and ultrastructure of the skin of rainbow trout was carried out, especially associated with growth and sexual maturation. An increase in the thickness of the dermis and epidermis and a decrease in the number of mucous cells were noted in the males, together with an infiltration of mononucleated cells in both sexes during sexual maturation. The structure of the gills and thymus was also examined. In the ultrastructural studies special attention was paid to lymphocyte-like and macrophage-like cells, which were interpreted as infiltrating elements. Presumed macrophages, with or without melanin granules, were seen crossing the basement membrane, which reinforced the idea of a dermal-epidermal traffic. Phosphotungstic-acid staining was applied to ultrathin sections of the epidermis to evaluate the distribution of complex carbohydrates. Among other localizations, positive staining was observed in mucous cells and in the membrane of large vesicular bodies in macrophages and filament-containing cells. The possibility of such structures being lysosomes is discussed. Immunoglobulin-containing cells were located in the skin through immunocytochemical methods. PAP and immunogold labelling, in semithin and ultrathin sections. Rabbit serum anti-trout Ig was used and the details of its production are given. In the epidermis, lymphocyte-like cells showed a degree of intracytoplasmic labelling, but this was not sufficient to draw conclusions as to whether these cells were antibody-producing or not. Some mucous cells stained positively. To evaluate how bath vaccination influenced the production of antibody in the skin, a group of juvenile fish were vaccinated inti-Vibrio anguillarum. Immunocytochemical studies were undertaken, using an immuno-sandwich technique, to locate specific antibody anti-Vibrio anguillarum. Finally, a discussion is presented of the involvement of the skin in specific immune reactions. It seems to be a tissue fully equipped for antigen-trapping, where mucous cells could be responsible for the transport, or even the synthesis, of antibody into the skin surface, to be incorporated into the mucus. The lack of protection shown by the skin of sexually mature males of certain salmonid species is considered and thought possibly due to the decrease in mucus production under hormonal influence and thereby a decrease in available immunoglobulins at the body surface.en_GB
dc.language.isoenen_GB
dc.publisherUniversity of Stirlingen_GB
dc.subject.lcshRainbow trouten_GB
dc.subject.lcshRainbow trout Physiologyen_GB
dc.subject.lcshFishes Physiologyen_GB
dc.titleMorphological and cytochemical studies on the skin of rainbow trout, Salmo gairdneri, Richardsonen_GB
dc.typeThesis or Dissertationen_GB
dc.type.qualificationlevelDoctoralen_GB
dc.type.qualificationnameDoctor of Philosophyen_GB
Appears in Collections:Aquaculture eTheses

Files in This Item:
File Description SizeFormat 
Peleteiro-Thesis.pdf29.85 MBAdobe PDFView/Open


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.